Coding

Part:BBa_K2074030

Designed by: Ruicheng Dai   Group: iGEM16_FAFU-CHINA   (2016-10-09)


Cry10Aa(Codon optimization)+Extended FMDV 2A+Cyt2(Codon optimization) 800px-T--FAFU-CHINA--partsclones.png

Cry10Aa and Cyt2 are proteins from Bacillus thuringiensis (Bt),which have toxicity to the larvae of mosquito.We used 2A system to express two proteins at the same time in Chlamydomonas reinhardti. These two proteins express at the same time can enhance the toxicity to the larvae of mosquito.

Fig.1 Cry10Aa and Cyt2 Toxins Group
Fig.2 The gel electrophoresis result of Cry10Aa+FMDV 2A+Cyt2

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 368
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 32
    Illegal BglII site found at 2282
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 181
    Illegal NgoMIV site found at 223
    Illegal NgoMIV site found at 280
    Illegal NgoMIV site found at 784
    Illegal NgoMIV site found at 1460
    Illegal NgoMIV site found at 2116
    Illegal NgoMIV site found at 2416
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 202
    Illegal SapI.rc site found at 924


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